Fig. 5.

Cytotoxic activity of CD19-CAR-NK92 cells despite inhibition of TRAIL. A Specific lysis (%) of Daudi and Karpas422 cells by reference CD19-CAR-NK92 cells with (gray) and without (white) anti-TRAIL antibody (5 μg/ml). B Specific lysis (%) of Daudi and Karpas422 cells by FasL- & Prf1-ko CD19-CAR-NK92 cells with (gray) and without (white) anti-TRAIL antibody (5 μg/ml). A + B Cells were co-incubated for 4 h. Two cell clones were used, each assay was performed in triplicates and the results were combined in one data group. Values are expressed as means of multiple determination ± SEM. P values were calculated by unpaired t test