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. 2023 Jul 21;69(4):81. doi: 10.1007/s10344-023-01709-8

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© The Author(s) 2023

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Fig. 2 — Double-color FISH with U1 and U2 snDNA probes. The U1 probe (red) reveals one clear signal (= a pair of homologous chromosomes) in a X. tropicalis, c X. pygmaeus, d X. allofraseri, and e X. laevis, while the same FISH shows two signals, each within homoeologous chromosomes in b X. calcaratus and f X. muelleri. The U2 (green) probe shows one signal in a X. tropicalis, c X. pygmaeus, d X. allofraseri, and e X. laevis, while the U2 probe shows two signals, each of them within homoeologous chromosomes in b X. calcaratus and f X. muelleri. Green and red arrows correspond to the U2 and U1 repeat loci, respectively. Painting probes were used for identification of chromosomes 1 (green) and 8 (red) in a X. tropicalis. Chromosomes were counterstained with DAPI in blue/gray. Scale bars represent 10 µM