TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| S17-1 | RP4-2 (Tc::Mu) (Km::Tn7) integrated into the chromosome | 39 |
| JM83 | Host for pUC plasmids | 45 |
| R. capsulatus | ||
| B10S | Spontaneous Smr mutant of R. capsulatus B10 | 17 |
| KS36 | ΔnifHDK::Spc | 46 |
| R438I | modA::Gm, insertion mutant of KS36 | 46 |
| Xan-17 | moeA::Tn5 | 20 |
| R487 | ΔntrY::Gm, deletion mutant of KS36 | This work |
| R488 | ClaI::Gm, insertion mutant of KS36 | This work |
| R507 | ΔmoeA/ntrY::Gm, deletion mutant of KS36 | This work |
| R509I/II | moeA::Km, insertion mutant of KS36 | This work |
| Plasmids | ||
| pNF3 | nifH promoter plasmid | 28 |
| pPHU231 | Broad-host-range vector | 31 |
| pPHU235 | Broad-host-range lacZ fusion vector | 10 |
| pFR400 | pPHU231 carrying nap genes of R. sphaeroides | 31 |
| pSL157 | pPHU231 carrying xdhABC expressed from nifH promoter derived from pNF3 | This work |
| pWKR500 | 7.3-kb EcoRI fragment carrying moeA cloned into pUC8 | This work |
| pWKR513 | moeA-lacZ fusion vector; EcoRI-PstI fragment carrying the 5′ end of moeA cloned into pPHU235 | This work |