Fig. 2.

Loss of Nck1 or Nck2 delays ductal outgrowth and total branch area at 5 and 8 weeks. A Representative wholemount images of L4 mammary gland outgrowth in WT, Nck1KO, and Nck2KO mice at 5 weeks, 8 weeks, and 12 weeks of age. Scale bar 1 mm. B Ductal branch area was quantified using FIJI where a perpendicular line was drawn at the back of the lymph node (LN) and the full branching region extending from here was outlined (n = 5–10 per genotype). C Ductal outgrowth was quantified by averages measuring from the back of the lymph node (LN) to the 3–4 furthest extensions of the ductal network (n = 5–10 mice of each genotype per timepoint). B,C, 1KO: Nck1KO, 2KO: Nck2KO, scale bar 1 mm; * adjusted P < 0.05; ** P < 0.01; **** P < 0.0001; at each time point by one-way ANOVA with post-hoc Dunnett’s test of Nck1KO and Nck2KO compared to WT