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. 2023 Jul 15;201(2):329–338. doi: 10.1007/s10549-023-07016-9

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© The Author(s) 2023

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Fig. 1 — a Hematoxylin phloxine saffron (HPS) stain of DCIS grade 1, with small and monotonous epithelial nuclei. 20X magnification. b HPS stain of DCIS grade 2, small and monotonous epithelial nuclei with comedo-type necrosis. 20X magnification. c HPS staining of DCIS grade 3 enlarged and pleomorphic epithelial nuclei. 20X magnification. d IHC staining of DCIS showing strong nuclear estrogen receptor (ER) expression. 20X magnification. e IHC stain of DCIS showing strong nuclear progesterone receptor (PR) expression. 20X magnification. f IHC staining of DCIS showing strong and complete membranous expression (3+) of human epidermal growth factor receptor 2 (HER2). 20X magnification. g IHC staining of DCIS showing nuclear Ki67 expression. 20X magnification