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. 2023 Apr 16;72(8):2623–2633. doi: 10.1007/s00262-023-03437-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — anti-B7-H3 mAb biodistribution in a NSG mice model. A Schematic representation of experimental design. PBS or different doses of anti-B7-H3 mAb (clone 8H9) labeled with Alexa Fluor 647 is administrated by intraperitoneal injection (IP) to NSG mice-bearing 143B tumors. B and D Tumors are imaged at different time points using IVIS imaging system. Representative IVIS image and mean ± SD of average radiant efficiency are shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by two-way ANOVA with Tukey (B) and Sidak (D) post hoc test. C B7-H3 expression on ex vivo tumor cells is evaluated by flow cytometry at 72 h. Solid histograms (left) represent the expression of B7-H3 on 143B cells. One representative profile per group is shown. Graph (right) shows the mean fluorescence intensities (MFI) mean ± SD of at least 3 mice. **, P < 0.01; by one-way ANOVA with Tukey post hoc test. E Ex vivo imaging of OS tumors and the organs indicated after 72 h of PBS or anti-B7-H3 Alexa Fluor 647 administration. F Quantification of total radiant efficiency of ex vivo imaging (mean ± SD) *, P < 0.05 by two-way ANOVA with Sidak post hoc test