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. 2023 Apr 16;72(8):2623–2633. doi: 10.1007/s00262-023-03437-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — CAR T cell migration in vitro to osteosarcoma cell lines. Chemotaxis assay is performed adding starving CAR T cells in transwell chambers over osteosarcoma cell lines preincubated (red) or not (blue) with anti-B7-H3-FITC mAb. Basal and positive control (FBS) are included. After 4 h, CAR T cells migrated are collected and counted by flow cytometry. Migration index mean ± SD of independent experiments from three different donor is shown