Fig. 4. GOLPH3 knockdown decreases Golgi stress and fragmentation.

FL83B and HK-2 cells were transfected with Con siRNA or GOLPH3 siRNA for 24 h, and then treated with LPS (1 µg/ml) for 8 h. A, B Western blot analysis was performed to evaluate transfection efficiency of GOLPH3 siRNA after 24 h. C, D After transfection and LPS treatment, the Golgi morphology of cells were examined. Representative images of immunofluorescence staining of GM130 (green) and GOLPH3 (red), and the quantification of cells with fragmented Golgi from each group are shown (n = 3). Scale bar, 20 µm. The data are presented as mean ± SEM. Two-tailed Student’s t-test (in A, B) and One-way ANOVA, followed by Bonferroni’s multiple comparisons (in C, D) were used, *p < 0.05 versus Con siRNA alone, and ^#p < 0.05 vs LPS with Con siRNA.