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. 2023 Jul 21;13:11802. doi: 10.1038/s41598-023-38830-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of claudin-5/7 and Fas in the colon are altered by switching to a high fiber diet. (A) A high fat diet decreased the expression of claudin-5, claudin-7 and fas; whereas, switching to a fiber diet increased its expression, as determined by western blot. Each blot is the representative of three blots. (B) The relative protein band intensity of claudin-5 (*P < 0.05), claudin-7 (***P < 0.001) and Fas determined by Image J and statistically analyzed by one-way ANOVA (n = 3).