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. 2023 Jul 21;13:11802. doi: 10.1038/s41598-023-38830-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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High fat/high protein diet decreased NeuN expressing enteric neurons in the colon muscularis mucosa. (A) The muscularis mucosa of the colon of mice treated with standard, antibiotic, fiber and carbohydrate diets had good NeuN expressing enteric neurons compared to colon from mice fed with a high fat/high protein diet. However, the mucosal layer of the colon from high fat/high protein diet had high expression of the NeuN enteric neurons and GFAP. The figures are representative of three images. (B) The colon of mice on a high fat high protein diet had low levels of NeuN expression in the muscularis mucosa as quantitated by CTCF analysis (CTCF analysis, n = 3 mice tissues per treatment) (**P < 0.01, as determined by one-way ANOVA).