Figure 3.

Bin1 mAb treatment increased the expression of the tight junction protein claudin-5 and the stem cell marker BMI-1 of the colon. (A) Mice were fed with different diets and induced UC with DSS (n = 5 mice per treatment) for 6 days. The animals were fed drinking water for 24 h and later subjected to Bin1 immunotherapeutic treatment. All the mice fed with fiber diet died in 4 days after Bin1 immunotherapy. The colon of mice was removed 7 days after Bin1 immunotherapy followed by western blotting. Experiments were done in duplicate as samples were highly sensitive to degradation. (B) Relative protein band intensity of Claudin 5 and BMI1 (**P < 0.01, as determined by one-way ANOVA).