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. 2023 Jul 21;13:11802. doi: 10.1038/s41598-023-38830-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of diet switching from a high fat/high protein diet to a high fiber diet. Mice were fed with a high fat/high protein diet for two months and switched to a fiber diet for 40 days. (A) The weight of the animals decreased upon switching to a fiber diet. (B) The weight of fecal pellets increased upon switching to a fiber diet. (C) Comparison of weight of animals on different diets 40 days after switching to a fiber diet. (D) The length of colon increased after switching to a fiber diet. The colon was harvested 40 days after switching from a high fat/high protein diet to a fiber diet. (**P < 0.01, ***P < 0.001, ****P < 0.0001 as determined by t test). All results are expressed as Mean ± SD (n = 5 mice per treatment).