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Expression of the AhpC, Nox-1, and Nox-2 proteins in S. mutans wild-type strain GS-5 before and 60, 120, and 240 min after exposure to air on TYG medium. Each protein was analyzed by immunoblotting as described in Materials and Methods. Each lane was loaded with 5 μg of protein from the corresponding extract. The leftmost lane shows the purified enzymes applied as controls (from top to bottom, 50 ng of AhpC, 100 ng of Nox-1, and 100 ng of Nox-2).
