TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference |
|---|---|---|
| Strains | ||
| S. mutans | ||
| GS-5 | Wild type, serotype c | 31 |
| E22 | Δnox-1 Emr | This study |
| N2E | Δnox-2 Emr | This study |
| N2S | Δnox-2 Spcr | This study |
| B1 | ΔahpC Emr | This study |
| BEE | ΔahpC Δnox-1 Emr | This study |
| BES | ΔahpC Δnox-1 Spcr | This study |
| N2S-B1 | Δnox-2 ΔahpC Emr Spcr | This study |
| N2S-E22 | Δnox-1 Δnox-2 Emr Spcr | This study |
| N2E-BES | ΔahpC Δnox-1 Δnox-2 Emr Spcr | This study |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 9 |
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB)/F′ [traD36 proAB+ lacIqlacZΔM15] | 30 |
| K12 | ahpC+ ahpF+ (parent of TA4315) | 27 |
| TA4315 | ΔahpC ΔahpF | 27 |
| Plasmids | ||
| pUC18 | AprlacPOZ′ | 30 |
| pUC119 | AprlacPOZ′ | 30 |
| pTSE | Emr 0.9-kb BamHI | 2 |
| pSPC1 | Spcr 1.1-kb BamHI | 15 |
| pMS1 | 1.9-kb EcoRI fraction from λHS-1 containing nox-1 in pUC119 | This study |
| pNOX1-H | 1.6-kb HindIII fragment containing nox-1 in pKK223-3 | This study |
| pAN119 | 2.5-kb EcoRI-HindIII fragment from λHS-1 containing ahpC and nox-1 in pUC119 | This study |
| pSSW61 | 5.6-kb SacI fragment containing nox-2 in pMW118 | 19 |
| pB1 | ahpC::Emr | This study |
| pE22 | nox1::Emr | This study |
| pN2E | nox2::Emr | This study |
| pN2S | nox2::Spcr | This study |
| pBEE | ahpC::Emr::nox-1 | This study |
| pBES | ahpC::Spcr::nox-1 | This study |