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. 2023 Jul 21;13:11789. doi: 10.1038/s41598-023-39003-x

Figure 1.

Figure 1

Expression of CALD1, COCH, and CCN2. (A) Expression of CALD1, COCH, and CCN2 in the E96 cochlea. Among them, only CALD1 expression was observed in the developing periotic mesenchymal tissue. (B) Expression of CALD1, COCH, and CCN2 in the E115 cochlea. While CALD1 expression was widely observed in the spiral ligament fibrocytes, only weak COCH expression was detected at this stage (arrow in B). No CCN2 expression was observed in spiral ligament fibrocytes at this stage. (C,D) Expression of CALD1, COCH, and CCN2 in the E120 cochlea. At this stage, CALD1 expression is widely observed in spiral ligament fibrocytes, and a relatively broader expression of COCH was detected compared with E115. However, CCN2 expression could not be detected at E120. (E,F) Expression of CALD1, COCH, and CCN2 in the P0 cochlea. At P0, both COCH and CCN2 expressions were observed ubiquitously in spiral ligament fibrocytes. CALD1 expression in type II, IV, and V fibrocytes was reduced and restricted to type I and III at this stage. Nuclei were counterstained with Hoechst (blue). Scale bar 50 µm in (A,D), 100 µm in (B,C,E,F). StV stria vascularis, I type I fibrocytes, II type II fibrocytes, III type III fibrocytes, IV type IV fibrocytes. (A–F) Basal turns.