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. 2023 Jul 21;13:11775. doi: 10.1038/s41598-023-38881-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Single-cell CNA profiling of rare cells detected in SCLC peripheral blood samples. (A) CNA profile of representative single rare cells from each SCLC patient and their Landscape-stained cell images; CK in red, Vim in white, CD45/CD31 in green, and DAPI in blue. The rare cell type of each cell is annotated with color labels at the top right. (B) CNA profiles of sequenced cells from UM-001 stained by Landscape assay. (C) CNA profiles of sequenced cells from UM-001 stained by EpCAM-targeted assay. EpCAM expression for each cell is shown at the bottom of the heatmap. The rare cell group of each cell in B and C are annotated with different color labels at the top left of each heatmap. CNA gains are shown in red, neutrals in white, and losses in blue.