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. 2023 Jul 21;13:11819. doi: 10.1038/s41598-023-38241-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(I) Screening of lipase producers on phenol red dye containing plates. (A) CIG-23H^T (test culture). (B) Pseudozyma antarctica MTCC 2706 (positive control). (C) E. coli MTCC 1610 (negative control). (D) Uninoculated (control). (E) CIG-23H^T (without substrate). (II) Screening of lipase producers on cresol red dye containing plates. (A) CIG-23H^T (test culture). (B) Pseudozyma antarctica MTCC 2706 (positive control). (C) E. coli MTCC 1610 (negative control). (D) Uninoculated (control). (E) CIG-23H^T (without substrate). (III) Screening of lipase producers on BTB dye containing plates. (A) CIG-23H^T (test culture). (B) Pseudozyma antarctica MTCC 2706 (positive control). (C) E. coli MTCC 1610 (negative control). (D) Uninoculated (control). (E) CIG-23H^T (without substrate). (IV) Screening of lipase producers on Rhodamine B dye containing plates. (A) CIG-23H^T (test culture). (B) Pseudozyma antarctica MTCC 2706 (positive control). (C) E. coli MTCC 1610 (negative control). (D) Uninoculated (control). (E) CIG-23H^T (without substrate). (V) Screening of lipase producers in phenol red dye containing flasks. (A) Uninoculated (control). (B) CIG-23H^T (test culture). (C) Pseudozyma antarctica MTCC 2706 (positive control). (D) E. coli MTCC 1610 (negative control). (E) CIG-23H^T (without substrate). (VI) Screening of lipase producers in cresol red dye containing flasks. (A) Uninoculated (control). (B) CIG-23H^T (test culture). (C) Pseudozyma antarctica MTCC 2706 (positive control). (D) E. coli MTCC 1610 (negative control). (E) CIG-23H^T (without substrate). (VII) Screening of lipase producers in BTB dye containing flasks. (A) Uninoculated (control). (B) CIG-23H^T (test culture). (C) Pseudozyma antarctica MTCC 2706 (positive control). (D) E. coli MTCC 1610 (negative control). (E) CIG-23H^T (without substrate). (VIII) Screening of lipase producers in Rhodamine B dye containing flasks. (A) Uninoculated (control). (B) CIG-23H^T (test culture). (C) Pseudozyma antarctica MTCC 2706 (positive control). (D) E. coli MTCC 1610 (negative control). (E) CIG-23H^T (without substrate).

(I) Screening of lipase producers on phenol red dye containing plates. (A) CIG-23H^T (test culture). (B) Pseudozyma antarctica MTCC 2706 (positive control). (C) E. coli MTCC 1610 (negative control). (D) Uninoculated (control). (E) CIG-23H^T (without substrate). (II) Screening of lipase producers on cresol red dye containing plates. (A) CIG-23H^T (test culture). (B) Pseudozyma antarctica MTCC 2706 (positive control). (C) E. coli MTCC 1610 (negative control). (D) Uninoculated (control). (E) CIG-23H^T (without substrate). (III) Screening of lipase producers on BTB dye containing plates. (A) CIG-23H^T (test culture). (B) Pseudozyma antarctica MTCC 2706 (positive control). (C) E. coli MTCC 1610 (negative control). (D) Uninoculated (control). (E) CIG-23H^T (without substrate). (IV) Screening of lipase producers on Rhodamine B dye containing plates. (A) CIG-23H^T (test culture). (B) Pseudozyma antarctica MTCC 2706 (positive control). (C) E. coli MTCC 1610 (negative control). (D) Uninoculated (control). (E) CIG-23H^T (without substrate). (V) Screening of lipase producers in phenol red dye containing flasks. (A) Uninoculated (control). (B) CIG-23H^T (test culture). (C) Pseudozyma antarctica MTCC 2706 (positive control). (D) E. coli MTCC 1610 (negative control). (E) CIG-23H^T (without substrate). (VI) Screening of lipase producers in cresol red dye containing flasks. (A) Uninoculated (control). (B) CIG-23H^T (test culture). (C) Pseudozyma antarctica MTCC 2706 (positive control). (D) E. coli MTCC 1610 (negative control). (E) CIG-23H^T (without substrate). (VII) Screening of lipase producers in BTB dye containing flasks. (A) Uninoculated (control). (B) CIG-23H^T (test culture). (C) Pseudozyma antarctica MTCC 2706 (positive control). (D) E. coli MTCC 1610 (negative control). (E) CIG-23H^T (without substrate). (VIII) Screening of lipase producers in Rhodamine B dye containing flasks. (A) Uninoculated (control). (B) CIG-23H^T (test culture). (C) Pseudozyma antarctica MTCC 2706 (positive control). (D) E. coli MTCC 1610 (negative control). (E) CIG-23H^T (without substrate).