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. 2023 Jul 21;3:77. doi: 10.1038/s43705-023-00283-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Taxonomic identification of 150 bp cpn60 ASV from saliva (A), vagina (B) and infant stool (C) microbiomes by wateredBLAST (abscissa, % identity) and the classifier (ordinate, confidence at species rank). Density of data points is indicated by red contour lines. Broken blue lines indicate two thresholds: ≥55% sequence identity for wateredBLAST (the established cut-off for non-cpn60 sequences) and 0.8 for the classifier (the default confidence threshold). Number (percent) of ASV in each quadrant of the plots are shown in (D).