Table 1.
Bacterial strains and plasmids.
| Name | Relevant features | References |
|---|---|---|
| Bacterial strains | ||
| C-1a | Prototroph | 42 |
| C-5691 | C-1a Δpnp-751 | 43 |
| C-5692 | C-5691 (DE3) | 13 |
| C-6001 | C-1a Δpnp::PNPT1Ec | This work |
| C-6009 | C-1a pnp-His tag | This work |
| C-6011 | C-1a Δpnp::PNPT1Ec-His tag | This work |
| Plasmids | ||
| pCP20 | Flippase encoding plasmid | 41 |
| pKD4 | kanR encoding plasmid | 41 |
| pKD46 | λRED plasmid | 41 |
| pET28b-H6-hPNPase | hPNPase coding sequence with the E. coli codon usage (PNPT1Ec) and N-ter 6 × His tag | 14 |
| pETD-hPNP | Carries IPTG-inducible PNPT1Ec cloned in pETDuet-1 (Novagen) | This work |