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. 2023 Jul 21;6:766. doi: 10.1038/s42003-023-05105-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Generation of DE-SC culture from CL tissues. b Left, representative bright field images of DE-SC colonies and BrdU staining in Ctrl and Med1 cKO. Bar = 10 mm. Right, quantification of colony size and BrdU positive cells/colony are shown as fold changes (cKO/Ctrl) with standard deviations (error bars); in both comparisons (n = 6–9, t-test p < 0.01). c Biological processes associated with loss of Med1 in DE-SCs as identified by Ingenuity Pathway Analysis (IPA) on microarray gene expression data. d Heatmap showing differential gene expressions of epidermal and hair-related genes in Med1 cKO vs Ctrl incisor tissues (CL and Sec in vivo) and cultured Med1 cKO vs Ctrl DE-SCs (third lane); n = 3 for all samples and average values for each comparison are shown. e Upstream regulators responsible for biological process induced by loss of Med1 in DE-SCs as identified by IPA on microarray data as used in c. f Mechanistic network representation for TP53/63 pathways in induced in Med1 lacking DE-SC culture regulating epidermal fate driving AP-1 factors; upregulated genes are in orange and direct relationships are shown by solid lines. Reproducibility was confirmed by two independent cultures, and representative data are shown.