Fig. 6. Loss of Med1 expands the super-enhancer landscape in CLH and CLT tissues.

a Schematic representation for the isolation of CLH (stem cells) and CLT (stem cell progenies) from Med1 cKO and littermate Ctrl mandibles for ChIP-seq against H3K27ac to compare actively transcribed genomic regions. b GREAT based GO analysis for genes associated with differential H3K27ac peaks to elucidate biological processes affected by Med1 loss in CLT tissues. c Number of super-enhancers in Ctrl (blue) and cKO (pink) tissues. We compare typical and super-enhancers associated with epidermal and hair-related genes before and after loss of Med1 in CLH and CLT tissues. As a comparison, data from skin-derived keratinocytes are included. d ChIP-seq profiles on mouse genome (mm10) for H3K27ac occupancy for the Fosl2 gene, the super-enhancer in the cKO sample is underscored by a blue line. e Most enriched transcription factor binding motifs found in super-enhancers formed in Med1 cKO compared to Ctrl in CLT tissues; statistical significances are shown as -Log₁₀(p-values). All the ChIP-seq data are averages of duplicates conducted in 2 different litters of Med1 cKO and littermate controls, in which CL tissues are pooled from 2–4 mice for each group (total 4–8 cervical loop tissues) in each ChIP experiment.