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. 2023 Jul 21;13:11790. doi: 10.1038/s41598-023-35401-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of immunization of two clustered subtypes. (A) Box plots showed the mRNA expression of immune checkpoints in two clusters (*P < 0.05; **P < 0.01; ***P < 0.001). (B) Stacked bar chart showed the percentage of immune cells in 1811 patients. (C) Box plots were used to display the distribution of the cell proportions calculated by the CIBERSORT algorithm of immune cells between the two clusters (*P < 0.05; **P < 0.01; ***P < 0.001). (D) Box plot of the distribution of immune cell expression between the two clusters as calculated by the ssGSEA algorithm (*P < 0.05; **P < 0.01; ***P < 0.001). (E–J) Box plots were created to visualize the distribution of the Stromal Score, Immune Score, ESTIMATES Score, and Tumor Purity, which were calculated by the ESTIMATE algorithm between the two clusters in the GSE45547 (E), GSE49710 (F), GSE73517 (G), GSE120559 (H), E-MTAB-828 (I) and TARGET (J) datasets.