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. 2023 Jul 21;13:11787. doi: 10.1038/s41598-023-38607-7

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Camera calibration and calculating the scaling factor. (a) Pinhole camera model, (b) schematic diagram of scaling factor calculation for converting the pixel unit to length unit (mm). $D_{mm}$ and $D_{pixel}$ are the physical distance of two points in millimeters and the distance of the same points in the image pixel, respectively.