Skip to main content
. 2023 Jul 21;14(7):457. doi: 10.1038/s41419-023-05952-4

Fig. 6. Lactate aggravates SAKI by promoting excessive mitochondrial fission.

Fig. 6

A–E Effects of lactate on the protein expression of Fis1, DRP1, MFN1, and MFN2 in HK-2 cells (n = 6). F–G The mitochondrial morphology of HK-2 cells was observed under confocal microscope by MitoTracker staining. The images were analyzed by the Mitochondrial Network Analysis (MiNA) plugin of ImageJ software (n = 17). Scale bars, 5 μm. H Lactate promoted the LPS-induced Fis1-DRP1 interaction in HK-2 cells. (I-J) Fis1 knockdown attenuated the reduction in cell viability and ATP depletion induced by lactate+LPS stimulation in HK-2 cells (n = 5). K–L Mdivi-1 (10 μM) administration attenuated the reduction in cell viability and ATP depletion induced by lactate+LPS stimulation in HK-2 cells (n = 5). M, N Flow cytometry assessment after MitoSOX staining showed that Mdivi-1 reduced the LPS+lactate-induced mtROS elevation in HK-2 cells (n = 6). O–Q The effects of Mdivi-1 on the expression of the antiapoptotic protein Bcl-2 and the proapoptotic protein Bak in HK-2 cells (n = 6). R, S Mdivi-1 reduced CLP+lactate-induced elevation of SCr and BUN in mice (n = 5). Data are mean ± SD; *P, #P, &P < 0.05.