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. 2023 Jul 21;14(7):457. doi: 10.1038/s41419-023-05952-4

Fig. 7. Fis1 K20 is the key site by which lactate mediates Fis1 lactylation and exacerbates SAKI.

Fig. 7

A Protein multiple sequence alignment analysis of Fis1 20 sites. B Verification of the specificity of the Fis1 K20la antibody by dot blot experiment. C Immunoblotting with the Fis1 K20la antibody after Fis1 IP in HK-2 cells. D, E Recognition of Fis1 lactylation in HK-2 whole-cell lysates by the Fis1 K20la antibody (n = 6). F Fis1 K20R mutation eliminated Fis1 K20la in HK-2 cells. G Fis1 K20R mutation inhibited the Fis1 interaction with DRP1 in HK-2 cells. H, I Effects of Fis1 WT and K20R plasmid overexpression on the mitochondrial network (n = 16). Scale bars, 5 μm. J, K Fis1 K20R mutation inhibited Fis1 WT-mediated mtROS overproduction (n = 6). L–N Fis1 K20R mutation inhibited the Fis1 WT-mediated Bcl-2 expression decrease and Bak expression increase (n = 6). Data are mean ± SD; *P, #P, &P < 0.05.