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. 2023 Jul 21;14:4407. doi: 10.1038/s41467-023-40037-y

Fig. 3. Dynamic epithelial proliferation in partial IRI kidneys.

Fig. 3

a Serial in vivo 2-photon microscopy of an ischemic region in a partial IRI CycB1-GFP reporter mouse kidney on days 0, 1, 2, and 3 after reperfusion shows increasing GFP expression (arrowheads) over time. Scale bar: 100 μm. b–d Volumetric quantification of GFP expression (% of total nuclei/ segment) across different experimental groups (b: sham and partial IRI), different partial IRI injury regions (c: Not-IR, IR, and Mid), and different nephron segments located in IR and Mid regions of partial IRI kidneys (d: PT-S1, PT-S2 and DCT/CD). Data from n = 3 sham and 9 partial IRI mice. Detailed information on tubule segment number per group is provided in Supplementary Table 1 (b, c) and Supplementary Table 2 (d); boxplot, line at median, edges at 25th and 75th percentiles, whiskers from min to max, individual points considered outliers. An overview of the same dataset, including expanded statistical comparison, is shown in Supplementary Fig. 5. Statistical test: linear mixed-effect model, p-values from two-sided tests (Supplementary Extended Statistics.j). #: significant difference within group when compared to respective day 0; #: p < 0.05; ##: p < 0.01; ###: p < 0.001. e Representative in vivo 2-photon image of epithelial GFP expression in an IR region of a CycB1-GFP kidney at 2 days after partial IRI. Scale bar: 75 μm.