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. 2023 Jul 21;14(7):453. doi: 10.1038/s41419-023-05934-6

Fig. 7. Effect of H-MW-HA on apoptosis and cleaved caspase-3 activation in the oral and intestinal mucosa induced by 5-fluorouracil (5-FU).

Fig. 7

5-FU was injected i.v. while H-MW-HA treatment (0.01%w/v) was daily administered orally in the drinking water as described in the method. Mice were sacrificed at day 16. The tongue and jejunum were excised, and sectioned. A Cleaved-caspases-3 immunostaining of tongue, B cleaved-caspases-3 immunostaining of intestinal tissue, C TUNEL stain of tongue tissue were performed (images at magnification 20X; scale bar represents 50 μm and apply to all panels). Black arrow heads point to the positive cell staining. The number of apoptotic cells in tongue mucosa (A, three different transverse sections/tongue mucosa, three representative mice/group), caspase-3-activated cells in tongue mucosa (B, six random fields of tongue mucosa/tongue section, three different transverse sections/tongue, three representative mice/group), and caspase-3-activated cells in intestinal mucosa (C, twelve full villus/section, four sections/mice intestine, three representative mice/group) were counted. Data are presented as the means ± SD. where, **p < 0.005, and ***p < 0.001.