Fig. 6.
Western blot analysis of PPARα, ACADM, ACCA1, ACSL1, ACSL4 and ACSL5. (A) Expression level of PPARα in liver detected by Western blot and semi-quantitative evaluation of PPARα/GAPDH relative expression. (B) Expression level of ACADM in liver investigated by Western blot and semi-quantitative evaluation of ACADM/β-actin relative expression. (C) Expression level of ACCA1 in liver examined by Western blot and semi-quantitative evaluation of ACCA1/β-actin relative expression. (D) Expression level of ACSL1 in liver tested by Western blot and semi-quantitative evaluation of ACSL1/β-actin relative expression. (E) Expression level of ACSL4 in liver detected by Western blot and semi-quantitative evaluation of ACSL4/β-actin relative expression. (F) Expression level of ACSL5 in liver tested by Western blot and semi-quantitative evaluation of ACSL5/β-actin relative expression. Lane1: the control group; Lane2: the TG189 mg/kg group; Lane3: the TG472.5 mg/kg group; Lane4: the TG189 mg/kg + GA20.25 mg/kg group; Lane5: the TG472.5 mg/kg + GA20.25 mg/kg group. *, the TG groups compared with the control group, p < 0.05; **, the TG groups compared with the control group, p < 0.01; #, the TG + GA group compared with the TG group at the same dose, p < 0.05; ##, the TG + GA group compared with the TG group at the same dose, p < 0.01.
