Table 1.
Purification yield rate of bacteriocin of L. sakei ZFM225.
Purification step | Volume (mL) | Total activity (IU) | Total protein (mg) | Specific activity (IU/mg) | Purification fold | Recovery (%) |
---|---|---|---|---|---|---|
CFS | 2000 | 532000 | 50000 | 10.64 | 1.00 | 100.00 |
ammonium sulfate precipitation | 60 | 135420 | 334 | 405.45 | 38.11 | 25.45 |
SP cation exchange | 45 | 49680 | 91 | 545.93 | 51.31 | 9.33 |
HPLC | 10 | 7840 | 10 | 784.00 | 73.68 | 1.47 |