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. 2023 Jun 27;30:16–26. doi: 10.1016/j.omto.2023.06.002

Figure 3.

Figure 3

Confirmation of Ras degradation on a microfluidic tumor-on-a-chip

(A) Western blot on MCF7 cell lysates after 2 days of treatment with 100 nM Ec1-ETA-RRSP or RRSP-DT-Ec1 on a 3D microfluidic tumor model. The control is untreated; n = 3, representing independent microfluidic devices. β-Actin (42 kDa) was used as a loading control. (B) Western blot quantitative analysis to determine the normalized Ras levels present in the MCF7 lysate. Results are shown as mean values. The error bars reflect the SEM. ∗∗∗p ≤ 0.001, based on a one-way ANOVA (n = 3). The Ras signal was normalized for the intensity of the control β-actin band intensity. (C) Immunofluorescence staining for pan-Ras in MCF7 cells after 2 days of treatment with 100 nM Ec1-ETA-RRSP or RRSP-DT-Ec1 on a 3D microfluidic system. The control is untreated; n = 3, representing independent microfluidic devices. Hoechst 33342 was used as nuclear staining. Scale bars, 100 μm. (D) Zoom-ins from (C). Arrowheads indicate examples of the apoptotic bodies formed. Scale bar, 25 μm.