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. 1999 Oct;181(19):5948–5957. doi: 10.1128/jb.181.19.5948-5957.1999

FIG. 1.

FIG. 1

Detection of the E. chrysanthemi EC16 KdgR and PecS proteins by electrophoresis mobility shift assays. The E. chrysanthemi 3937 pelD promoter-operator region (A) was incubated with 0, 2, 10, and 30 μg of E. chrysanthemi EC16 proteins contained in the 20 to 40% ammonium sulfate-saturated fraction (lane 1 to 4) or with 30 μg of E. chrysanthemi EC16 proteins contained in the 20 to 40% ammonium sulfate-saturated fraction followed by the addition of KdgR antibodies (lane 5). The E. chrysanthemi EC16 pelE promoter-operator region (B) was incubated with 0, 2, 10, and 30 μg of E. chrysanthemi EC16 proteins contained in the 55 to 70% ammonium sulfate-saturated fraction (lane 1 to 4) or with 30 μg followed by the addition of PecS antibodies (lane 5).