FIG. 6.

Cobinding of PecS and CRP on the EC16 pelE promoter-operator region. (A) Gel shift assays with the wild-type EC16 regulatory region. The concentration of the proteins used are indicated at the top. In lanes 6 and 8, the two proteins were added simultaneously; in lane 5, CRP was incubated 30 min before addition of PecS; in lane 7, PecS was incubated 30 min before addition of CRP. (B) Analysis of the effect of the modification in the CRP binding site 1 (CRP1⁻) on the PecS binding capacity by electrophoresis gel shift assays. WT, wild type.