TABLE 2.
Expression of the pelD::lacZ fusion in several backgroundsa
Strain | Genotype | β-Galactosidase sp act (nmol of product liberated min−1 mg of bacterial dry wt−1) | Derepression ratio |
---|---|---|---|
A3485 | Wild type | 13 | |
A3487 | kdgR | 644 | 50 |
A3486 | pecS | 42 | 3 |
A3489 | kdgR pecS | 2,581 | 200 |
Cultures were grown at 30°C for 14 h in M63 minimum medium supplemented with glycerol (2 g liter−1). β-Galactosidase activity reflects expression of the pelD::lacZ fusion. The results reported are averages of five independent experiments, each with a standard deviation of less than 20%.