Protocol to expand and CRISPR-Cas9 genomic edit murine MAIT cells for subsequent in vivo studies

. 2023 Jul 9;4(3):102419. doi: 10.1016/j.xpro.2023.102419

FACS/dissociation buffer

Reagent	Stock concentration	Final concentration	Amount
PBS	10×	1×	100 mL
BSA	N/A: lyophilized powder	0.5%	5 g
EDTA	0.5 M	2 mM	4 mL
Distilled H₂0	N/A	N/A	896 mL
Total	N/A	N/A	1 L

Store up to 2 months at 4°C.