Skin digestion buffer

Reagent	Stock concentration	Final concentration	Amount
Penicillin/Streptomycin	10,000 U/mL	100 U/mL	500 μL
Hepes	1 M	20 mM	1 mL
Sodium Pyruvate	100 mM	1 mM	500 μL
Non-essential Amino Acid	100×	1×	500 μL
β-mercaptoethanol	0.05 M	50 μM	50 μL
Liberase TL	5 mg/mL	0.25 mg/mL	2.5 mL
DNAse I	10 mg/mL	0.5 mg/mL	2.5 mL
RPMI 1640 GlutaMAX	N/A	N/A	qsp 50 mL
Total	N/A	N/A	50 mL

Store without liberase TL and DNAse I up to 2 months at 4°C. Liberase TL and DNAse I should be added to the medium the day of the experiment.