REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies (each lot of antibody should be titrated to ensure accurate staining)
CD44 (BV605)	BioLegend	Cat#103047; RRID: AB_2562451
CXCR6 (PETR)	BioLegend	Cat#151117; RRID: AB_2721700
MR1:5-OP-RU tetramer (PE)	NIH Tetramer Core Facility
MR1:6-FP (unlabeled)	NIH Tetramer Core Facility
TCRβ (APC-Cy7)	BioLegend	Cat#109220; RRID: AB_893626
Chemicals, peptides, and recombinant proteins
Anti-CD16/CD32 (Clone 2.4G2)	Institut Curie, produced in house
5-OP-RU	Institut Curie, produced in house	2
Alt-R S.p. HiFi Cas9 Nuclease V3	IDT	Cat#1081061
Anti-PE microbeads	Miltenyi	Cat#130-048-801
β-mercaptoethanol	Sigma	Cat#M3148
Bovine serum albumin	Sigma	Cat#A7906
Brefeldin A	Sigma	Cat#B6542
CO2 independent medium	Gibco	Cat#18045088
DAPI	Sigma	Cat#MBD0015
Debris removal solution	Miltenyi	Cat#130-109-398
Dispase	Corning	Cat#354235
DNAse 1	Roche	Cat#10104159001
EDTA	Gibco	Cat#15575-038
Fetal calf serum	Eurobio	Cat#CVFSVF00-01
HEPES	Gibco	Cat#15630-056
Liberase TL	Sigma	Cat#5401020001
Live and dead AQUA	Thermo Fischer Scientific	Cat#L34957
Non-essential amino acid	Thermo Fisher	Cat#11140050
PBS	Eurobio Scientific	Cat#CS1PB501-01
Penicillin/streptomycin	Gibco	Cat#15140-122
RPMI 1640 GlutaMAX	Gibco	Cat#61870036
Sodium Pyruvate (NaPyr)	Thermo Fisher	Cat#11360070
Critical commercial assays
Solution P3 Primary Cell 4DNucleofector X kit S	Lonza	Cat#VAXP-3032
Experimental models: Organisms/strains
Cd3ε−/−	Institut Curie	3
CD45.1/1, CD45.1/2 or CD45.2/2 B6-MAITCAST	Institut Curie	4,5
Oligonucleotides
Alt-R CRISPR-Cas9 crRNA : Mm_CXCR6.1_AA : /AltR1/rCrU rGrUrA rCrGrA rUrGrG rGrCrA rCrUrA rCrGrA rGrUrU rUrUrA rGrArG rCrUrA rUrGrC rU/AltR2/	IDT	N/A
Alt-R® CRISPR-Cas9 tracrRNA	IDT	Cat#1072534
Software and algorithms
Cytoflex software (CytExpert)	Beckman Coulter (V2.4)	https://www.beckman.fr/flow-cytometry/research-flow-cytometers/cytoflex/software
FlowJo	BD (V10.8.0)	https://www.flowjo.com/
Other
Biopsy punch (4Ø)	Stiefel	Cat#600210
Biopsy punch (5Ø)	Stiefel	Cat#600118
Coheban	3M	Cat# CO1583S
Cyanoacrylate adhesive	Sigma-Aldrich	Cat#Z105899
Cytoflex LX	Beckman Coulter	Cat#C00445
Dermal Betadine	Mylan	Cat#852
Hair removal cream	Veet	Cat#EA_3108955
LS columns	Miltenyi	Cat#130-042-401
Silicone sheet (5 mm thick)	Grace Bio-Labs	Cat#GBL664581-5EA
TEGADERM film dressing	3M	Cat#1626W
VICRYL 6-0 sutures	Ethicon	Cat#V302H