| Antibodies |
|
| Anti-mouse CD45 (Clone ID: 30-F11) APC-Cy7 (1:200) |
BioLegend |
103116; AB_312981 |
| Anti-mouse F4/80 (Clone ID: BM8) PE (1:133) |
BioLegend |
123110; AB_893486 |
| Anti-mouse B220 (Clone ID: RA3-B62) APC (1:100) |
BioLegend |
103212; AB_312997 |
| Anti-mouse CD11b (Clone ID: H1/70) BV 650 (1:200) |
BioLegend |
101259; AB_2566568 |
| Anti-mouse CD4 (Clone ID: RM4-5) Pacific Blue (1:200) |
BioLegend |
100531; AB_493374 |
| Anti-mouse CD8a (Clone ID: 53-6.7) PE-Cy7 (1:200) |
BioLegend |
100722; AB_312761 |
| Anti-mouse Siglec-F (Clone ID: E50-2440) PerCP-Cy5.5 (1:100) |
BD Pharma |
565526; AB_2739281 |
| Anti-mouse CD11c (Clone ID: N418) PE-Cy5 (1:133) |
BioLegend |
117316; AB_493566 |
| Anti-mouse Ly6G (Clone ID: 1A8) Alexa Fluor 488 (1:100) |
BioLegend |
127626; AB_2561340 |
| Anti-mouse Ly6C (Clone ID:HK1.4) BV 785 (1:100) |
BioLegend |
128041; AB_2565852 |
|
| Virus strain |
|
| RSV-A2 (Lot# 70040896) |
ATCC |
VR1540 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Isoflurane |
Akron Inc. |
NDC 59399-106-01 |
| Phosphate buffered saline (PBS) |
Hyclone |
SH30256.02 |
| EDTA 0.5 M solution |
RPI Research |
E14000-250.0 |
| Fetal bovine serum (FBS) |
Hyclone |
SH30396.03IH25-40 |
| Bovine serum albumin (BSA) 30% solution |
Sigma-Aldrich |
A3299 |
| Dextrose |
Fisher Chemicals |
D16-500 |
| Heparin sodium salt powder |
Sigma-Aldrich |
H3393-25KU |
| 4% Paraformaldehyde |
N/A |
N/A |
| 10% Formalin |
Sigma-Aldrich |
HT501128 |
| Ultra-pure water |
N/A |
N/A |
|
| Critical commercial assays |
|
| LIVE/DEAD Fixable Aqua Dead cell stain kit (1:200) |
Thermo Fisher Scientific |
L34966 |
|
| Experimental models: Cell lines |
|
| Hep2 cells |
ATCC |
CCL-23 |
|
| Experimental models: Organisms/strains |
|
| C57BL/6J mice, age:(P7) 7 days old neonates, sex: male/female |
Jackson Laboratories |
000664 |
|
| Software and algorithms |
|
| FlowJo |
BD Biosciences |
N/A |
| Prism 9 |
GraphPad |
N/A |
|
| Other |
|
| Small animal heating pad |
Amazon |
B00JHK375E |
| 3× LED Magnification lamp |
Amazon |
B08J4CWXYP |
| Mini coolers |
Heathrow Scientific |
120073 |
| Oxygen cylinder |
N/A |
N/A |
| Manual pipette |
Alphapette |
A-10 |
| Weighing balance |
N/A |
N/A |
| Tissue Lyser II |
Qiagen |
N/A |