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. 2023 Jul 9;4(3):102434. doi: 10.1016/j.xpro.2023.102434
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-mouse CD45 (Clone ID: 30-F11) APC-Cy7 (1:200) BioLegend 103116; AB_312981
Anti-mouse F4/80 (Clone ID: BM8) PE (1:133) BioLegend 123110; AB_893486
Anti-mouse B220 (Clone ID: RA3-B62) APC (1:100) BioLegend 103212; AB_312997
Anti-mouse CD11b (Clone ID: H1/70) BV 650 (1:200) BioLegend 101259; AB_2566568
Anti-mouse CD4 (Clone ID: RM4-5) Pacific Blue (1:200) BioLegend 100531; AB_493374
Anti-mouse CD8a (Clone ID: 53-6.7) PE-Cy7 (1:200) BioLegend 100722; AB_312761
Anti-mouse Siglec-F (Clone ID: E50-2440) PerCP-Cy5.5 (1:100) BD Pharma 565526; AB_2739281
Anti-mouse CD11c (Clone ID: N418) PE-Cy5 (1:133) BioLegend 117316; AB_493566
Anti-mouse Ly6G (Clone ID: 1A8) Alexa Fluor 488 (1:100) BioLegend 127626; AB_2561340
Anti-mouse Ly6C (Clone ID:HK1.4) BV 785 (1:100) BioLegend 128041; AB_2565852

Virus strain

RSV-A2 (Lot# 70040896) ATCC VR1540

Chemicals, peptides, and recombinant proteins

Isoflurane Akron Inc. NDC 59399-106-01
Phosphate buffered saline (PBS) Hyclone SH30256.02
EDTA 0.5 M solution RPI Research E14000-250.0
Fetal bovine serum (FBS) Hyclone SH30396.03IH25-40
Bovine serum albumin (BSA) 30% solution Sigma-Aldrich A3299
Dextrose Fisher Chemicals D16-500
Heparin sodium salt powder Sigma-Aldrich H3393-25KU
4% Paraformaldehyde N/A N/A
10% Formalin Sigma-Aldrich HT501128
Ultra-pure water N/A N/A

Critical commercial assays

LIVE/DEAD Fixable Aqua Dead cell stain kit (1:200) Thermo Fisher Scientific L34966

Experimental models: Cell lines

Hep2 cells ATCC CCL-23

Experimental models: Organisms/strains

C57BL/6J mice, age:(P7) 7 days old neonates, sex: male/female Jackson Laboratories 000664

Software and algorithms

FlowJo BD Biosciences N/A
Prism 9 GraphPad N/A

Other

Small animal heating pad Amazon B00JHK375E
3× LED Magnification lamp Amazon B08J4CWXYP
Mini coolers Heathrow Scientific 120073
Oxygen cylinder N/A N/A
Manual pipette Alphapette A-10
Weighing balance N/A N/A
Tissue Lyser II Qiagen N/A