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. 2023 Jul 4;65:102807. doi: 10.1016/j.redox.2023.102807

Table 1.

SNPs identified for SELENBP1 and mutants generated in this work.a

Position Amino acid change SNP ID (if available) Se bindingb MTO activityb Cu bindingb Reference Other missense changes/SNP ID (if different)
5d Cys→Ser (↓) This work
8d Cys→Ser (↓) This work
57 Cys→Ser = (↓) (↑) This work
73 His→Phe n. d. c ↓↓ = This work
74 His→Phe n. d. c ↓↓ = This work
80 Cys→Ser rs1043849 (↑) (↑) This work Cys→Arg rs762533745
83e Cys→Ser rs750650780 = (↑) = This work Cys→Phe rs1180081309
137 His→Phe n. d. c ↓↓ This work His→Gln rs752623236
140 His→Phe n. d. c ↓↓ This work His→Arg
His→Leu rs201591413
141 Cys→Ser rs371854240 = (↑) = This work Cys→Tyr
189 Asp→Asn n. d. c ↓↓ This work
225 Gly→Trp rs758495626 n. d. ↓↓ n. d. [7,9] Gly→Arg
252 Glu→Gln n. d. c ↓↓ This work Glu→Asp rs762922279
329 His→Tyr n. d. ↓↓ n. d. [7,9] His→Arg
His→Leu rs765409789
a

Source: Genome Aggregation Database (gnomAD) browser, v. 2.1.1 (https://gnomad.broadinstitute.org/).

b

The symbols are: ↑ (increase by approx. 50% or more); (↑) (weak increase, less than 50%); = (no effect, less than 20% change); (↓) (weak decrease, less than 50%); ↓ (decrease by approx. 50% or more); ↓↓ (decrease by approx. 90% or more); n.d., not done.

c

TXRF measurements determine both Cu and Se content in parallel. Whereas Cu is present in freshly isolated SELENBP1 (see Fig. 2), Se is not (see Fig. 1). As no selenite loading of samples analyzed for changes in Cu content was performed, there was no Se detected in these samples – hence, the analysis of Se binding was “not done”.

d

As Cys5,8 double mutant.

e

As Cys80,83 double mutant.