. 2023 Jul 4;9(7):e17655. doi: 10.1016/j.heliyon.2023.e17655

Table 7.

Biofilm formation of test bacteria on polystyrene in thickened Marinades 1 and 2 when stored under different conditions. * indicates a significant difference (P < 0.05) in biofilm formation after ANOVA testing.

Species	Temp. (°C)	M1	M1T	M2	M2T
S. enterica ser. Typhimurium	4 °C	++	++	+++	+++
S. enterica ser. Typhimurium	37 °C	+++	+++	++++	+++++
L. innocua	4 °C	++	++	+++	++
L. innocua	37 °C	++	++++ *	+++	+++
E. coli	4 °C	++	++	+++	++
E. coli	37 °C	+++	+++	+++	+++
B. subtilis	4 °C	++	++	+++	++
B. subtilis	37 °C	++	++	+++	+++++ *

‘+’ and ‘-‘ indicate the growth levels as determined by O_D600 spectraphotometric values where.

“—" = < 0.05. “+” = < 0.15. “++” = < 0.35. “+++” = < 0.7. “++++” = < 1. “+++++” = > 1.