FIG. 3.

Bordetella ⁵⁵Fe-ferric alcaligin transport assays. The rate of uptake of the ⁵⁵Fe label of ⁵⁵Fe-ferric alcaligin by iron-starved B. bronchiseptica wild-type strain B013N (squares), ΔfauA mutant BRM18 (diamonds), and ΔfauA mutant BRM18 carrying wild-type fauA plasmid pBB24 (circles), using an alcaligin concentration of 5 μM, together with 3.3 μM ⁵⁵Fe(III) is shown over a 7-min period of time. Mean ⁵⁵Fe counts per minute ± standard deviations (n = 3) are plotted as a function of time.