FIG. 4.

Production of Bordetella FauA polypeptides, using a bacteriophage T7 RNA polymerase/promoter expression system in E. coli. Intrinsically labeled plasmid-encoded FauA proteins were resolved by SDS-PAGE and visualized by autoradiography. Whole-cell lysates of E. coli K38(pGP1-2) containing p3Z63 (B. pertussis 3.8-kb PstI fauA fragment; Bp fauA), p3Z48 (B. bronchiseptica 3.8-kb PstI fauA fragment; Bb fauA), p3Z69 (B. bronchiseptica 3.8-kb PstI ΔfauA fragment deleted of the 255-bp internal fauA region; Bb ΔfauA), and pGEM3Z (plasmid vector control; Control) are shown. U, uninduced; I, induced for expression of T7 promoter-directed genes by temperature shift and in the presence of rifampin. Positions of marker proteins are given along with their apparent molecular masses in kilodaltons. The radiolabeled FauA products are indicated by arrows.