FIG. 5.

SDS-PAGE analysis of iron-regulated FauA outer membrane protein production in B. bronchiseptica. FauA outer membrane protein production by wild-type B. bronchiseptica B013N, ΔfauA mutant BRM18, and ΔfauA mutant BRM18 carrying wild-type fauA plasmid pBB24 was analyzed by SDS-PAGE. Outer membrane proteins were visualized by staining gels with Coomassie blue dye. +, outer membrane proteins from iron-replete cells; −, outer membrane proteins from iron-depleted cells. Arrowheads indicate positions of the 79-kDa FauA protein in the wild-type B013N and BRM18(pBB24) outer membrane samples. Positions of marker proteins are given along with their apparent molecular masses in kilodaltons.