TABLE 1.
Bordetella strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Reference or source |
|---|---|---|
| B. bronchiseptica strains | ||
| B013N | fauA+, nalidixic acid-resistant derivative of swine isolate strain B | 6 |
| BRM17 | fauA1::Kanr mutant derivative of B013N, with an insertion of a 1.2-kb BamHI kanamycin resistance cassette into the BglII restriction site in fauA | This study |
| BRM18 | ΔfauA2 mutant derivative of B013N, with a nonpolar, in-frame deletion of 255 bp of fauA coding sequences | This study |
| BRM11 | ΔalcR1 mutant derivative of B013N | 11 |
| Recombinant plasmids | ||
| pCP1.11 | B. pertussis strain UT25 fauA+ cosmid, source of sequenced fauA DNA, RK2 origin, RK2-Mob+, RK2-Tra−, Tcr | 30 |
| pBRM6 | 20-kb fauA+ SalI genomic DNA fragment of B. bronchiseptica alcC mutant BRM6 cloned in pGEM3Z, Apr, ColE1 origin | 6 |
| p3Z44 | 7-kb B. bronchiseptica fauA+ EcoRI-SalI DNA fragment subcloned from pBRM6 to pGEM3Z, Apr, ColE1 origin | This study |
| p3Z48 | 3.8-kb B. bronchiseptica fauA+ PstI DNA fragment subcloned from p3Z44 to pGEM3Z, Apr, ColE1 origin | This study |
| p3Z49 | As p3Z48 except opposite orientation of the 3.8-kb fauA+ PstI DNA insert fragment | This study |
| pBB24 | 3.8-kb B. bronchiseptica fauA+ PstI DNA fragment subcloned from p3Z44 to pBBR1MCS, Cmr | This study |
| pBB24ΔfauA | As pBB24 except carrying the B. bronchiseptica ΔfauA2 mutant allele on the PstI DNA insert fragment | This study |
| p3Z63 | 3.8-kb B. pertussis UT25 fauA+ PstI DNA fragment subcloned from pCP1.11 to pGEM3Z, Apr, ColE1 origin | This study |
| p3Z69 | As p3Z48 except carrying the B. bronchiseptica ΔfauA2 mutant allele on the PstI DNA insert fragment | This study |
| pBB24/mini-Tn5 lacZ1 | As pBB24 except carrying the B. bronchiseptica φ(fauA′-lacZ+)1 allele generated by mini-Tn5 lacZ1 transposon mutagenesis, Cmr, Kanr | This study |