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. 2023 Jul 8;9(7):e18119. doi: 10.1016/j.heliyon.2023.e18119

Fig. 4.

Fig. 4

UV–Vis absorbance spectra from 240 to 400 nm. Absorbance in the 260 nm range of the aqueous formulations suggests there may be RNA present in the biological BECC preparations. Panel A shows the absorbance spectra for BECC438b and BECC438s, panel B shows the spectra for BECC470b and BECC470s, and panel C shows the spectra for MPL and PHAD. Panel D shows the results of a dot blot assay of all six lipid A analogues after incubation with RiboGreen. The observed fluorescence for BECC438b and BECC470b suggests the presence of RNA. The original dot blot without cropping is shown in Suppl. Fig. S2.