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. 2023 Jun 28;26(7):107225. doi: 10.1016/j.isci.2023.107225

Figure 4.

Figure 4

Col12a1 KO mice show a malformed femoral trochlear groove and M. rectus femoris alterations at P7

(A) Representative images of μCT volume renderings of femora (gray), M. rectus femoris (green), patella and ligament (red) from P7 old WT and Col12a1 KO mice.

(B) Representative images of μCT volume renderings of femora from WT and Col12a1 KO mice. Arrows point at the femoral trochlear groove, malformed in KO (n = 4 samples for WT group, n = 5 samples for KO group, see Figure S4).

(C) μCT 3D morphometric analysis shows decreased length and volume of M. rectus femoris in P7 old Col12a1 KO mice (n = 4 samples for WT group, n = 5 samples for KO group). Unpaired two-tailed Student’s t test, ∗∗∗, p < 0.001. Error bars are mean ± SD.

(D) μCT 2D morphometric analysis along the length of the Lugol’s stained M. rectus femoris from 0% (proximal end) to 100% (distal end) show differences between the Col12a1 KO group (n = 5 samples) when compared to the WT group (n = 4 samples) in muscle perimeter and muscle area. Line graphs represent the means ± SEM. Unpaired two-tailed Student’s t test was used to compare means between WT and Col12a1 KO groups. The graphical heatmap displays statistical differences at specific matched locations along the length of the femur. Red p ≤ 0.000–0.001, yellow p ≤ 0.001–0.01, green p ≤ 0.01–0.05 and blue p ≥ 0.05.