Figure 3.

Kctd19 mutant mice recapitulate the NOA phenotype of individuals homozygous for the KCTD19 variants

(A) Testis of 2-month-old WT, Kctd19^m1/m1 and Kctd19^m2/m2 mice. Scale bar indicates 4 mm.

(B) Testis to body weight ratio of 2-month-old WT, Kctd19^m1/m1 and Kctd19^m2/m2 mice. Data are obtained from three or four mice for each genotype. The significance was determined via one-way ANOVA followed by Dunnett’s test. ∗∗∗∗p < 0.0001.

(C) H&E staining of testicular and epididymal sections from adult WT, Kctd19^m1/m1 and Kctd19^m2/m2 mice. Black boxes indicate metaphase cells. Scale bars indicate 50 μm.

(D) Immunofluorescence staining of testicular sections from adult WT, Kctd19^m1/m1 and Kctd19^m2/m2 mice with PNA (red) and an antibody against H3S10p (green). The nuclei were stained with Hoechst 33342 (blue). Scale bars indicate 20 μm.