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. 2023 Jun 28;26(7):107193. doi: 10.1016/j.isci.2023.107193

Figure 4.

Figure 4

Kctd19 mutant mice show abnormal individualization of meiotic metaphase I chromosomes

(A) Immunofluorescence staining of metaphase I chromosome spreads from WT and Kctd19m1/m1 testes with an antibody against SYCP3 (green). The chromosomes were stained with Hoechst 33342 (gray). In MMI of WT mice, most of the bivalents have a distinct outline and can be easily identified, while in MMI of mutant mice, most of the bivalents are clustered together and difficult to be distinguished individually. Scale bars indicate 5 μm.

(B) Proportion of meiotic metaphase I (MMI) spermatocytes with clustered chromosomes in (A). n, the number of MMI spermatocytes scored from three mice per genotype. The significance was determined via unpaired Student’s t test. ∗∗∗p < 0.001.

(C) Immunofluorescence staining of MMI chromosome spreads from WT and Kctd19m1/m1 testes with antibodies against CAP-H (red) and SYCP3 (green). The chromosomes were stained with Hoechst 33342 (gray). Scale bars indicate 5 μm.