Figure 5.

Kctd19 mutations result in decreased Kctd19 mRNA levels and loss of KCTD19 protein in testes

(A) Quantitative real-time PCR analysis of Kctd19 mRNA levels in testes from adult WT, Kctd19^m1/m1 and Kctd19^m2/m2 mice. Actb was used as the internal control. Data are from three experiments (three mice per genotype in total). The significance was determined via one-way ANOVA followed by Dunnett’s test. ∗∗∗p < 0.001, ∗∗p < 0.01.

(B) Western blotting with testis lysates from adult WT, Kctd19^m1/m1 and Kctd19^m2/m2 mice using an anti-KCTD19-N antibody. Red arrows indicate the target proteins. β-actin was used as the loading control.