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. 2023 Jun 24;26(7):107210. doi: 10.1016/j.isci.2023.107210

Figure 1.

Figure 1

In situ structure of the C. burnetii T4SS

(A and B) Slices through electron cryotomograms of LCV C. burnetii cells highlighting the presence of T4SS particles (black arrows). Scale bar 100 nm.

(C) Enlargements of the T4SS particles shown in A (top panel) and B (lower panel). Scale bar 10 nm.

(D) A slice thorough an electron cryotomogram of a lysed C. burnetii cell illustrating the presence of multiple top views of T4SS particles (white arrows). Scale bar 100 nm. An enlargement of a top view of a T4SS particle is shown in the white-boxed area.

(E and F) Slices through the subtomogram averages of T4SS of C. burnetii at pH 7.2 (left) and pH 4.75 (right). Dashed yellow line indicates a composite of two averages obtained with different masks (see STAR Methods) concatenated together. Scale bar 10 nm.

(G) A cross section through the T4SS OM complex (at the level indicated with the orange arrow in E) showing 13-fold symmetry. OM = outer membrane, IM = inner membrane.