Figure 3.

Multilayered cytoplasmic membrane invaginations in C. burnetii SCVs

(A–E) Slices through electron cryotomograms of C. burnetii cells (purified from infected vero cells 28 dpi) highlighting the presence of multilayered cytoplasmic membrane invaginations (orange arrows) with zoom-ins of these invaginations shown in the enlargements at the lower right corner of each panel. The white arrows in panel C point to a membrane of presumably the host vacuole still attached to the OM of C. burnetii cell. Scale bar is 100 nm.

(F) Lower panel: average density profile taken along the area indicated between the yellow lines in the upper panel highlighting the equidistant spacing of ∼10 nm between the different layers of the inner membrane invaginations.